Variable lipoprotein haemagglutinin (vlhA) gene sequence typing of mainly DutchMycoplasma synoviaeisolates: comparison withvlhAsequences from Genbank and with amplified fragment length polymorphism analysis

Amplified Fragment Length Polymorphism Analysis in Strain Typing and Identification of Listeria and Clostridium Species

Experimental versus in silico fluorescent amplified fragment length polymorphism analysis of Mycobacterium tuberculosis: improved typing with an extended fragment range.

Whole-genome fingerprinting fluorescent amplified fragment length polymorphism (FAFLP) data were compared with in silico data for the sequenced strains of Mycobacterium tuberculosis (H37Rv and CDC1551). For this G+C-rich genome, many predicted fragments were not detected experimentally. For H37Rv, only 108 (66%) of the 163 predicted EcoRI-MseI fragments between 100 and 500 bp were visualized in...

Use of Single-enzyme Amplified Fragment Length Polymorphism for Typing Clostridium Perfringens Isolated from Diarrheic Piglets

Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine pres...

Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus cereus

We examined 154 Norwegian B. cereus and B. thuringiensis soil isolates (collected from five different locations), 8 B. cereus and 2 B. thuringiensis reference strains, and 2 Bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (AFLP). We employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fr...

Amplified-fragment length polymorphism analysis: the state of an art.

P. H. M. SAVELKOUL,* H. J. M. AARTS, J. DE HAAS, L. DIJKSHOORN, B. DUIM, M. OTSEN, J. L. W. RADEMAKER, L. SCHOULS, AND J. A. LENSTRA Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, Amsterdam, DLO-State Institute of Quality Control of Agricultural Products Centre De Born, Wageningen, Novartis Seeds bv, Section Biotechnology, Enkhuizen, Department...